It is known that in-situ hybridization can be used to determine the cryptologic and histological location of the nucleic acid of interest

 

  Fluorescent in Situ Hybridization (FISH) Probe is a subatomic cytogenetic technique that visualizes genetic materials using fluorescent probes. These probes are molecules that absorb a specific wavelength of light and emit light when they bind to a specific DNA/RNA sequence. They are used to detect structural and numerical chromosomal abnormalities, monitor therapeutic drugs, and identify rare genetic diseases. FISH probes that are commonly used include locus-specific, Alphoid/centromeric repeat, and whole chromosome probes. They have several advantages, including high sensitivity and accuracy in recognizing targeted sequences, direct application to both metaphase and interphase nuclei, and accurate visualization of hybrid signals at the single-cell level.

  One of the key factors driving the market growth is the increasing prevalence of various genetic and chronic disorders. Furthermore, the rising demand for In Vitro Diagnostics (IVD) testing and targeted therapies around the world is propelling the market forward. When compared to traditional cytogenetic (cell gene) tests, FISH tests can detect minute genetic changes that are normally missed under the microscope. As a result, these probes are widely used for cancer and genetic disorder diagnosis, prediction of outcomes, and clinical management. Furthermore, various technological advancements, such as the development of FISH probes with higher sensitivity and accuracy, are contributing to the market's positive outlook. Other factors, such as improving healthcare infrastructure, particularly in developing countries, and extensive research and development (R&D) in biotechnology, are expected to drive the market even further.

  In-situ Hybridization (ISH) is a well-established set of methods for detecting and visualizing specific nucleic acid sequences in whole organisms, cytological preparations, and tissue sections. Over the several decades of its use, the technique has seen refinements and a significant increase in applications, and it is still routinely used in laboratories where visualization of gene expression within the tissue of concern is required.

 

  In-situ hybridization is known to allow for the cryptologic and histologic localization of the nucleic acid of interest. While the techniques have matured significantly in the current scenario, the application of most In-situ Hybridization techniques has been severely limited due to their inability to detect samples with low copies of DNA and RNA. Advances in recent years have resulted in the development of a number of strategies to help improve the sensitivity of ISH techniques, such as signal detection after hybridization or amplification of the target nucleic acid sequence before ISH begins.